PHENOTYPIC CHARACTERISTICS OF CELLS DERIVED FROM PRECURSORS OF HUMAN-MELANOMA
- 1 January 1983
- journal article
- research article
- Vol. 43 (11), 5502-5508
Abstract
Of 66 specimens from benign melanocytic nevi, including common acquired and congenital nevi, Spitz tumors (epithelioid cell nevi) and melanocytic nevi with dysplasia, 57 could be grown in tissue culture. The cultured cells were identified as melanocytes by the presence of premelanosomes and melanosomes. Cells from 28 of 32 nevus cultures grew in an anchorage-independent way in soft agar with a colony-forming efficiency between 0.001-1%. Clones derived from single cells and soft agar-selected colonies showed marked phenotypic heterogeneity, but all had a limited life span and did not undergo transformation in culture. These cells were nontumorigenic in nude mice. Cultured nevus cells expressed antigens present on melanoma but absent on normal fibroblasts and/or melanocytes as tested with monoclonal antimelanoma antibodies. The antimelanoma antibodies bound equally well to dysplastic, congenital and common acquired nevi. Antigens are released by nevus cells similar to melanoma cells.This publication has 17 references indexed in Scilit:
- MALIGNANT MELANOMA IN RENAL-TRANSPLANT RECIPIENTSThe Lancet, 1981
- INDUCTION OF ANCHORAGE-INDEPENDENT GROWTH IN HUMAN-FIBROBLASTS BY PROPANE SULTONE1981
- Short Communication: Carcinogen-induced anchorage-independent growth and in vivo lethality of human MRC-5 cellsCarcinogenesis: Integrative Cancer Research, 1981
- Dysplastic nevus syndrome: A phenotypic association of sporadic cutaneous melanomaCancer, 1980
- SPECIFIC IMMUNOREACTIVITY OF HYBRIDOMA-SECRETED MONOCLONAL ANTI-MELANOMA ANTIBODIES TO CULTURED-CELLS AND FRESHLY DERIVED HUMAN-CELLS1980
- ULTRAVIOLET LIGHT-INDUCED TRANSFORMATION OF HUMAN-CELLS TO ANCHORAGE-INDEPENDENT GROWTH1980
- Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies.Proceedings of the National Academy of Sciences, 1979
- Study of antibodies against human melanoma produced by somatic cell hybrids.Proceedings of the National Academy of Sciences, 1978
- Origin of familial malignant melanomas from heritable melanocytic lesions. 'The B-K mole syndrome'Archives of Dermatology, 1978
- Double Minute Chromosomes and the Homogeneously Staining Regions in Chromosomes of a Human Neuroblastoma Cell LineScience, 1977