Establishment of Long-Term Cultures of Ehrlich Ascites Tumor Cells.

Abstract

ELD hyperdiploid Ehrlich ascites tumor (chromosome mode 44) was successfully explanted from ICR/Ha Swiss mice into long-term culture for making morphologic comparisons with the in vivo tumor. Cells grow on a medium of Earle''s salts supplemented with fetal calf serum and bovine amniotic fluid. Concentrations of 2 to 40% human ascites fluid in combination with varying concentrations of fetal calf, calf, or horse serum in Earle''s medium or Earle''s basic salts did not support growth. Decreasing the concentration of fetal calf serum or amniotic fluid in the basic medium of established cultures retarded growth after 10 days; in two weeks, a large number of multinucleated giant cells and cells with vacuoles were observed. The modal chromosome number was 69-73 for the first 6 months; it then shifted to 67-75. Inoculation of tissue-cultured cells up to 12 months old induced tumor formation in mice.