Microfilaments and microtubules in calcium ionophore-induced secretion of lysosomal enzymes from human polymorphonuclear leukocytes.
Open Access
- 1 September 1978
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 78 (3), 769-781
- https://doi.org/10.1083/jcb.78.3.769
Abstract
Human peripheral blood leukocytes (PMN) were induced to release lysosomal enzymes by the calcium ionophore A23187 in the presence but not the absence of extracellular Ca++. Whereas secretion induced by particulate or immune stimuli is accompanied by an increase in visible microtubules and is inhibitable by colchicine, secretion induced by A23187 and Ca++ was not accompanied by an increase in microtubule numbers and was not inhibited by colchicine. Ca++ did not appear to regulate microtubule assembly in these cells since resting PMN had a mean of 22.3 .+-. 2.0 microtubules in the centriolar region as compared to 22.3 .+-. 1.1 in ionophore-treated cells and 24.9 .+-. 1.5 in cells exposed to ionophore and 1 mM Ca++. Bipolar filaments, 10 nm thick and 300-400 nm long, were numerous in the pericortical cytoplasm of cells exposed to both reagents. Microtubules in these cells were decorated with an electron-opaque fibrillar material. PMN exposed to A23187 and Ca++ were contracted in 2 directions at right angles to each other. Contractions parallel to the plasma membrane resulted in extensive plication of the cell membrane. The cytoplasm subjacent to the plicae contained dense filamentous webs. Plication was prevented by cytochalasin B or reversed by subsequent exposure to an endocytic stimulus such as zymosan. Contractions perpendicular to the plasma membrane, toward the cytocenter, resulted in the formation of vacuoles in normal PMN and of membrane invaginations in cytochalasin B-treated PMN. Whereas contractions parallel to the plasma membrane could occur in the absence of enzyme release (ionophore alone) and enzyme release could occur in the absence of such contractions (ionophore plus calcium plus cytochalasin B), contraction toward the cytocenter occurred in all experimental conditions in which significant enzyme release was obtained. Lysosomal enzyme secretion in PMN involves contractile movements of the plasma membrane toward the lysosomes rather than the reverse. These calcium-mediated contractile events are mediated by cytochalasin B-insensitive microfilaments but not by microtubule assembly.Keywords
This publication has 34 references indexed in Scilit:
- Changes in ionic movements across rabbit polymorphonuclear leukocyte membranes during lysosomal enzyme release. Possible ionic basis for lysosomal enzyme release.The Journal of cell biology, 1977
- Phagocytosis‐induced 45calcium efflux in polymorphonuclear leucocytesFEBS Letters, 1977
- Granule release by polymorphonuclear leukocytes treated with the lonophore A23187The Anatomical Record, 1977
- The contractile basis of amoeboid movement: V. The control of gelation, solation, and contraction in extracts from dictyostelium discoideumThe Journal of cell biology, 1977
- Influence of local anesthetics upon human polymorphonuclear leukocyte function in vitro. Reduction of lysosomal enzyme release and superoxide anion production.The Journal of Experimental Medicine, 1977
- Role of microtubule assembly in lysosomal enzyme secretion from human polymorphonuclear leukocytes. A reevaluation.The Journal of cell biology, 1977
- Lipid peroxidation and morphological changes in mammalian cells treated with the glutathione oxidant, diamide*1Experimental Cell Research, 1977
- Changes in membrane microviscosity associated with phagocytosis: effects of colchicine.Proceedings of the National Academy of Sciences, 1977
- Interactions of actin, myosin, and an actin-binding protein of rabbit pulmonary macrophages. III. Effects of cytochalasin B.The Journal of cell biology, 1976
- Metalloenzymes and myocardial infarction. II. Malic and lactic dehydrogenase activities and zinc concentrations in serum.1956