THE EFFECT OF SERUM FROM INSULIN-RESISTANT CASES ON THE COMBINATION OF INSULIN WITH THE RAT DIAPHRAGM 1

Abstract
The ability of human serum to influence the combination of insulin with the isolated rat diaphragm has been studied. The combina- tion of insulin with the diaphragm, as measured by its effect on glycogen synthesis, was found to be significantly decreased in the presence of serum from normal or diabetic individuals. Serum evidently contains substances which combine with or inactivate insulin. Serum from insulin-resistant patients appears to contain greatly increased amts. of such substances.