Inhibition of Pancreatic Secretion by the Carbonic Anhydrase Inhibitor 2-Acetylamino-1,3,4-Thiadiazole-5-Sulfonamide, Diamox (#6063)

Abstract

Animals were provided with a duodenostomy for cannulation of the pancreatic duct, according to the technic of Thomas et al. Doses as high as 60 mg, injected intraven., gave no evidence of toxicity. Pancreatic secretion following such injn. was reduced considerably, relative to suitable control observations. The max. degree of inhibition was 95% in terms of volume-rate of secretion, 60% for bicarbonate concn. and 97% for bicarbonate output. Evidence is adduced to support the idea that Diamox acts in these expts. by inhibiting pancreatic carbonic anhydrase directly, rather than secondarily through the inhibition of carbonic anhydrase in other organs. It may be concluded that pancreatic carbonic anhydrase plays an important role in the secretion of bicarbonate by this gland.