Spectrophotometric determination of hemoglobin oxygen saturation in hemolyzed whole blood by means of various wavelength Combinations

Abstract

The hemoglobin O2 saturation has been determined spectrophotometrically in hemolyzed whole blood in Klungsoyr and Stoa''s ultramicrocuvette with the wavelength combinations 576-560, 576[long dash]506 and 560[long dash]506 mu. On comparison of the analytical results, and investigation of the influence of increased content of bilirubin and lipids in the plasma, as well as increased content of COHb (carbon monoxide hemoglobin) in the blood of smokers, the combination 576[long dash]560 mu is found to be the most advantageous.