Measurement of the Cytoplasmic pH in Nitella translucens
- 1 April 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 59 (4), 664-666
- https://doi.org/10.1104/pp.59.4.664
Abstract
A comparison was made between the use of 2 types of pH microelectrode and the weak acid method for determining the cytoplasmic pH of N. translucens at an external pH of 6. There was good agreement between the value obtained with glass pH microelectrodes (7.54 .+-. 0.15 SE) and that obtained using the weak acid 5,5-dimethyloxazolidine-2,4-dione (7.42 .+-. 0.07 SE). Plastic-insulated antimony microelectrodes gave a significantly lower value (6.74 .+-. 0.15 SE) possibly due to disruption of the insulation by the cell wall. The addition of 1 mM NaN3 rapidly reduced the pH recorded by the glass pH microelectordes to about 5.3. A smaller change was observed using the weak acid method. The relevance of this observation to recent work on IAA transport is discussed.This publication has 5 references indexed in Scilit:
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