Perisomatic changes in the maturing hypoglossal nucleus after axon injury

Abstract

The perisomatic retrograde reactions to peripheral nerve injury during postnatal maturation were investigated by quantitative light and electron microscopy. The hypoglossal nerve was crushed, ligated or transected in 10 and 21 day postnatal (dpn) rats. Only crush injury was made in 7 dpn rats. Survival periods ranged from 3 to 40 days postoperative (dpo). Normal and sham operated animals of corresponding ages served as controls. A remarkable, transient response of neuronal somata in the young (7–10 dpn) rats, following all three types of axon injury, was the formation of excrescences which engulfed neuronal processes: boutons, dendrites, small neurites and a few myelinated axons in adjacent neuropil. The somal engulfment was rarely evident after nerve injury to 21 dpn rats. Bouton displacement from the somal surface, accompanied by glial incursion, followed each type of nerve injury but was less extensive and occurred later in the young rats. There seemed to be no association in the amount of boutons displaced from neuronal somata with the type of nerve injury for any of the three experimental age groups. However, the rate and intensity of the perineuronal glial reaction were related to the severity of the nerve injury in the older (21 dpn) but not in the younger (7–10 dpn) rats. Substantial loss of neurons occurred in the affected nucleus after each type of trauma to the young neurons. The degree of neuronal loss was related to the age and the volume of axoplasm disrupted. Only nerve transection in 21 dpn rats resulted in appreciable neuronal loss. The responses of axotomized neurons, their afferent axon terminals and the surrounding glia are quantitatively and qualitatively different in the hypoglossal nucleus of rats of increasing postnatal ages.