The in vitro enzymic hydroxylation of steroid hormones. 3. An investigation of the role of fumarate and pyridine nucleotides in the enzymic 11β-hydroxylation of 11-deoxycorticosterone
- 1 January 1956
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 62 (1), 29-33
- https://doi.org/10.1042/bj0620029
Abstract
It was shown that a specific requirement for fumarate for the 11[beta]-hydroxylation of steroids arises when ox-adrenocortical mitochondria, which carry the enzymes concerned, are subjected to damaging treatment such as pre-incubation without substrate in hypo-tonic media or acetone-drying. Such enzyme preparations also require triphosphopyridine nucleotide. Satisfactory explanations of the role of triphosphopyridine nucleotide and fumarate in the 11[beta]-hydroxylation reaction cannot be offered at present.Keywords
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