All three J-domain proteins of theEscherichia coliDnaK chaperone machinery are DNA binding proteins
- 25 February 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (9), 1935-1939
- https://doi.org/10.1016/j.febslet.2005.01.084
Abstract
DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well. Of special interest in this respect is DjlA, as this cytoplasmic protein is membrane bound and, as shown here, its affinity for DNA is extremely high. The finding that all the three J-proteins of DnaK are DNA binding proteins sheds new light on the cellular activity of these proteinsKeywords
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