Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein
- 1 April 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 22 (1), 1-8
- https://doi.org/10.1128/jvi.22.1.1-8.1977
Abstract
The Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolated nuclei of 2 EBV-transformed cell lines, [human Burkitt''s lymphoma] Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.This publication has 33 references indexed in Scilit:
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