Floral tissue ofPetunia hybrida(V30) expresses only one member of the chalcone synthase multigene family
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (13), 5229-5239
- https://doi.org/10.1093/nar/14.13.5229
Abstract
Twenty independent, petal-specific chalcone synthase (CHS) cDNA clones have been isolated from Petunia hybrida variety Violet 30 (V30). Sequence analysis shows that the largest of these clones contains the entire coding sequence. Using this clone in Southern blot analysis reveals the presence of multiple CHS gene copies in the genome of Petunia hybrida V30. Hybridization and sequence analysis of the CHS cDNA clones shows that they are all copied from a single mRNA species. This indicates the presence of only one transcriptionally active CHS gene in petals. Finally we report the identification, cloning and partial characterization of this gene.Keywords
This publication has 19 references indexed in Scilit:
- Metabolic changes in elicitor-treated bean cells. Selectivity of enzyme induction in relation to phytoalexin accumulationEuropean Journal of Biochemistry, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Rapid restriction mapping of DNA cloned in lambda phage vectorsGene, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Transformation of E. coli using homopolymer-linked plasmid chimerasBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Plasmid screening at high colony densityGene, 1980
- Highly purified “flavanone synthase” from parsley catalyzes the formation of naringenin chalconeArchives of Biochemistry and Biophysics, 1980
- [19] In Vitro packaging of λ and cosmid DNAMethods in Enzymology, 1979
- EK2 Derivatives of Bacteriophage Lambda Useful in the Cloning of DNA from Higher Organisms: The λgt WES SystemScience, 1977