Mammary development in mice: effects of hemihysterectomy in pregnancy and of litter size post partum

Abstract

Mice were hemihysterectomized on day 8 of pregnancy to reduce the number of feto-placental units. Fetal mortality was not affected by hemihysterectomy; mean single-pup weight at birth was increased when compared to sham-operated controls. Pregnant sham-operated and hemihysterectomized animals were killed on days 13 and 18 of gestation, and their mammary glands were analyzed for total DNA (DNAt) and RNA (RNAt). Both were significantly lower in the hemihysterectomized group on day 18, but not on day 13. Milk yield was assessed, by daily weighing of the litter in groups of sham-operated and hemihysterectomized lactating animals suckling 9 pups each. There was no difference in yield between the 2 groups. One group of sham-operated mice suckling 9 pups, 1 of hemihysterectomized mice suckling 9 pups and 1 of hemihysterectomized mice suckling 4 pups were killed on day 5 of lactation for mammary gland analysis. There was no significant difference in mammary weight or DNAt between the sham-operated and hemihysterectomized animals suckling 9 pups, although RNAt was still reduced in the latter. Mammary weight, DNAt and RNAt were all significantly lower in the hemihysterectomized group suckling 4 pups than in either of the other 2 groups. The less well developed mammary glands of mice which give birth to small litters are capable of compensatory growth during the 1st few days of lactation if a sufficiently strong suckling stimulus is given. Control of mammary development by the fetus during gestation and by the suckling young during early lactation are both mechanisms designed to ensure that milk yield is appropriate to the needs of the young.