Identification and characterization of putative transposable DNA elements in solanaceous plants and Caenorhabditis elegans.
- 12 September 1995
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (19), 8886-8890
- https://doi.org/10.1073/pnas.92.19.8886
Abstract
Several families of putative transposable elements (TrEs) in both solanaceous plants and Caenorhabditis elegans have been identified by screening the DNA data base for inverted repeated domains present in multiple copies in the genome. The elements are localized within intron and flanking regions of many genes. These elements consist of two inverted repeats flanking sequences ranging from 5 bp to > 500 bp. Identification of multiple elements in which sequence conservation includes both the flanking and internal regions implies that these TrEs are capable of duplicative transposition. Two of the elements were identified in promoter regions of the tomato (Lycoperiscon esculentum) polygalacturonase and potato (Solanum tuberosum) Win1 genes. The element in the polygalacturonase promoter spans a known regulatory region. In both cases, ancestral DNA sequences, which represent potential recombination target sequences prior to insertion of the elements, have been cloned from related species. The sequences of the inverted repeated domains in plants and C. elegans show a high degree of phylogenetic conservation. While frequency of the different elements is variable, some are present in very high copy number. A member of a single C. elegans TrE family is observed approximately once every 20 kb in the genome. The abundance of the described TrEs suggests utility in the genomic analysis of these and related organisms.Keywords
This publication has 21 references indexed in Scilit:
- Repeated Sequence Target Sites for Maternal DNA-Binding Proteins in Genes Activated in Early Sea Urchin DevelopmentDevelopmental Biology, 1994
- Plant bZIP proteins gather at ACGT elementsThe FASEB Journal, 1994
- The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzymeCell, 1993
- Endogenous retroviral sequences are required for tissue-specific expression of a human salivary amylase gene.Genes & Development, 1992
- The Tc2 Transposon ofCaenorhabditis elegansHas the Structure of a Self-Regulated ElementDNA and Cell Biology, 1992
- Identification of a novel murine IAP-promoted placenta-expressed geneNucleic Acids Research, 1991
- The mouse IgH 3′‐enhancerEuropean Journal of Immunology, 1991
- Retroviral long terminal repeat is the promoter of the gene encoding the tumor-associated calcium-binding protein oncomodulin in the ratJournal of Molecular Biology, 1989
- An ancient provirus has imposed androgen regulation on the adjacent mouse sex-limited protein geneCell, 1988
- Isolation of the transposable maize controlling elements Ac and DsCell, 1983