DIFFRACTOMETRIC MEASUREMENTS OF THE TONICITY-VOLUME RELATIONS OF HUMAN RED CELLS IN HYPOTONIC SYSTEMS
Open Access
- 20 May 1951
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 34 (5), 567-571
- https://doi.org/10.1085/jgp.34.5.567
Abstract
Red cell volumes in media sufficiently hypotonic to produce partial hemolysis have been measured with a high speed hematocrit and by an improved diffraction method. A comparison of the results shows that the upward departures from the linear relation based on the van't Hoff-Mariotte law are not observed when the volumes are measured diffractometrically. Downward departures are observed at low tonicities by both methods. These results provide strong evidence that the upward departures are due to the inclusion of semirigid ghosts in the column of packed red cells which is measured when the volumes are found with the hematocrit.Keywords
This publication has 3 references indexed in Scilit:
- THE TONICITY-VOLUME RELATIONS FOR SYSTEMS CONTAINING HUMAN RED CELLS AND THE CHLORIDES OF MONOVALENT CATIONSThe Journal of general physiology, 1949
- OSMOMETRIC BEHAVIOR OF NORMAL HUMAN ERYTHROCYTESJCI Insight, 1942
- A NEW FORM OF DIFFRACTOMETERThe Journal of general physiology, 1941