Applications of Fluorescence Correlation Spectroscopy

Abstract

The preceding paper by Douglas Magde has recounted the basic principles of Fluorescence Correlation Spectroscopy (FCS) as originally described (see Magde, Elson & Webb, 1972; Elson & Magde, 1974; Magde, Elson & Webb, 1974 Elson & Webb, 1975; referred to collectively as MEW), and has described the first application to chemical kinetics. In this paper I shall first illustrate the same principles of FCS with a simple graphical demonstration model based on the scheme for application to lateral diffusion in membranes as it was developed in our laboratory by Dr T. J. Herbert; I shall then proceed to discuss some current research in our group organized jointly with Professor E. L. Elson at Cornell.