Organization of turnip yellow mosaic virus investigated by neutron small angle scattering at 80 K: An intermediate state preceding decapsidation of the virion?
- 1 November 1993
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 17 (3), 223-231
- https://doi.org/10.1002/prot.340170302
Abstract
The organization of turnip yellow mosaic virus has been investigated by neutron small angle scattering at 300 K and 80 K in buffers containing various amounts of D2O. We confirm that in native virions, no substantial part of the RNA is located at a radius larger than ca. 100-110 Å, i.e., that there is very little interpretation of the RNA into the capsid. At 80 K, scattering curves do not depend much upon contrast, from 40% D2O to 100% D2O buffers, but are strongly affected by interparticle interference. We could, however, show that it is not the case for the subsidiary intensity maximum at q ∼ 0.06 Å−1. From the position of this maximum, we conclude that upon freezing, the radius of the capsid expands by c.a. 3.5% and the RNA penetrates deeply into the protein shell. Biological implications of this conformational change immediately preceding decapsidation are dicussed.Keywords
This publication has 28 references indexed in Scilit:
- Molecular basis of eye lens transparencyJournal of Molecular Biology, 1989
- Cryo-electron microscopy of vitrified specimensQuarterly Reviews of Biophysics, 1988
- Neutron scattering studies of the structure and assembly of spherical virusesMakromolekulare Chemie. Macromolecular Symposia, 1988
- Neutralization of poliovirus by polyclonal antibodies requires binding of a single IgG molecule per virionArchiv für die gesamte Virusforschung, 1986
- Studies on the Phenomenon of Turnip Yellow Mosaic Virus RNA Release by Freezing and ThawingJournal of General Virology, 1986
- Divalent ion-dependent reversible swelling of tomato bushy stunt virus and organization of the expanded virionJournal of Molecular Biology, 1982
- Comparative neutron small-angle scattering study of small spherical RNA virusesNature, 1977
- The neutron small-angle camera D11 at the high-flux reactor, GrenobleJournal of Applied Crystallography, 1976
- Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus: II. Electron microscopic studiesJournal of Molecular Biology, 1966
- Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virusJournal of Molecular Biology, 1966