Calcium dependent ATP losses in intact red blood cells without cellular accumulations of calcium

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Abstract
Intact human red blood cells incubated with ionophore A23187 and calcium develop a depletion of ATP that is dependent upon the concentrations of both A23187 and Ca. Incubations of fresh cells with 0.5 μm A23187 and concentrations of Ca at or below 70 μm produce a depletion of ATP without a net cellular uptake of Ca. In contrast, ATP-depleted cells display an ionophore-dependent cellular uptake of Ca, under identical conditions. A hypothesis is proposed that relates these ionophore-produced ATP depletions to active Ca extrusion by the Ca ATPase.