Quantitative Analysis of 5-Fluorouracil in Human Serum by High-Performance Liquid Chromatography
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 18 (1), 51-55
- https://doi.org/10.1080/00032718508066924
Abstract
A modified ion pair reverse-phase high-performance liquid chromatographic (HPLC) method for examining 5-fluorouracil levels in human serum was developed. The method employs the use of an internal standard and an organic extraction procedure. An isocratic mobile phase consisting of tetraethylammonium bromide, tetrabutylammonium hydrogen sulfate, methanol, sodium acetate, and phosphate buffer was used at a flow rate of 0.7 ml/min. 5-Fluorouracil was detected by UV absorption at a wavelength of 266 nm. The HPLC method described is a convenient and sensitive assay for examining 5-fluorouracil concentration in human serum.Keywords
This publication has 5 references indexed in Scilit:
- Reversed-phase ion-pair high-performance liquid chromatographic assay of 5-fluorouracil, 5′-deoxy-5-fluorouridine, their nucleosides, mono-, di-, and triphosphate nucleotides with a mixture of quaternary ammonium ionsJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- Schedule-dependent cytotoxicity of methotrexate and 5-fluorouracil in human colon and breast tumor cell lines.JCI Insight, 1980
- Enhanced 5-Fluorouracil Nucleotide Formation After Methotrexate Administration: Explanation for Drug SynergismScience, 1979
- High-performance liquid chromatographic analysis of 5-fluorouracil in plasmaJournal of Chromatography A, 1978
- SCHEDULE-DEPENDENT ANTITUMOR EFFECTS OF METHOTREXATE AND 5-FLUOROURACIL1977