Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains
Open Access
- 1 May 1996
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (9), 2527-2532
- https://doi.org/10.1128/jb.178.9.2527-2532.1996
Abstract
The insertion element IS1301 has been shown to mediate capsule phase variation in Neisseria meningitidis found in N. serogroup B by reversible insertional inactivation of the siaA gene. We have determined the target site specificity of this element by cloning and sequencing the insertion sites of 12 identical IS1301 copies found in N. meningitidis B1940. A target consensus core of 5'-AYTAG-3' was identified, with the central TA being duplicated following insertion. Additional features around the target sites, including extended palindromic symmetry, stem-loop formation, and the high incidence of AT tracts, indicate that other factors, such as DNA secondary structure, are involved in target recognition. The left inverted repeat of an IS1016-like element acts as a hot spot for insertion, with one insertion element combination located upstream of their gene. According to further sequence analysis, we were able to place IS1301 in the IS5 subgroup within the IS4 family of elements. A survey of 135 Neisseria strains indicated the presence of IS1301 in 27.9 to 33.3% of N. meningitides serogroup B, C, and W135 strains and in 86.7% of serogroup Y strains. IS1301 did not occur in serogroup A strains, in Neisseria gonorrhoeae, or in apathogenic Neisseria spp.Keywords
This publication has 42 references indexed in Scilit:
- Bacterial transposases and retroviral integrasesMolecular Microbiology, 1995
- Molecular analysis of the biosynthesis pathway of the α‐2,8 polysialic acid capsule by Neisseria meningitidls serogroup BMolecular Microbiology, 1994
- The IS4 family of insertion sequences: evidence for a conserved transposase motifMolecular Microbiology, 1993
- Cloning and nucleotide sequence of frpC, a second gene from Neisseria meningitidis encoding a protein similar to RTX cytotoxinsMolecular Microbiology, 1993
- Translational frameshifting in the control of transposition in bacteriaMolecular Microbiology, 1993
- Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic NeisseriaeFEMS Microbiology Letters, 1992
- Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic NeisseriaeFEMS Microbiology Letters, 1992
- Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidisMolecular Microbiology, 1992
- The Haemophilus influenzae capsulation gene cluster: a compound transposonMolecular Microbiology, 1991
- Functional similarities between retroviruses and the IS3 family of bacterial insertion sequences?Molecular Microbiology, 1990