Infectious DNA from Herpes Simplex Virus: Infectivity of Double-stranded and Single-stranded Molecules
- 1 December 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (12), 3621-3625
- https://doi.org/10.1073/pnas.70.12.3621
Abstract
The infectious units in native and alkalidenatured preparations of DNA of herpes simplex virus were characterized with respect to their sensitivity to Neurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. Infectious molecules in native preparations were resistant to N. crassa endonuclease, sedimented at 56 S, and were highly sensitive to shearing forces. After alkaline denaturation, infectious molecules became sensitive to the N. crassa enzyme, sedimented at 200 S, and were relatively resistant to shear. We conclude that both intact duplex molecules ([unk]100 x 10(6) daltons) and intact single strands ([unk]50 x 10(6) daltons) are capable of initiating productive infection.Keywords
This publication has 16 references indexed in Scilit:
- Physicochemical properties of the DNA of herpes virusesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Herpes vimplex virus: genome size and redundancy studied by renaturation kinetics.1971
- Size and composition of Marek's disease virus deoxyribonucleic acid.1971
- [Interactions between diethylaminoethyl-dextran and deoxyribonucleic acid].1969
- Conformational changes of single-stranded DNAJournal of Molecular Biology, 1969
- [Infectious power of Herpesvirus hominis DNA in cell culture].1969
- Herpes simplex virus DNAVirology, 1968
- Stages in the Replication of Bacteriophage X174 DNA in vivoCold Spring Harbor Symposia on Quantitative Biology, 1968
- [Morphologic and ultrastructural characteristics of cells of the epidermis of adult rabbits in tissue culture].1967
- Enhancement of infectivity of poliovirus RNA with diethylaminoethyl-dextran (DEAE-D)Archiv für die gesamte Virusforschung, 1965