Properties of the factor VIII after DDAVP (1-deamino-8-D-arginine vasopressin) infusion in normal subjects.

Abstract

The factor VIII response to DDAVP (1-deamino-8-D-arginine vasopressin) was examined in 6 healthy volunteers. Following i.v. DDAVP infusion each subject showed a marked increase in factor VIII procoagulant activity (VIII:C), a factor VIII related antigen (VIIIR:AG) and von Willebrand factor activity, but there were differences among augmentation index of each activity. Further investigations were performed to see whether there was a qualitative abnormality in the factor VIII after DDAVP infusion. Between factor VIII activities before and after DDAVP, there was no difference in thermostability of VVII:C in vitro, gel chromatographic pattern on Sepharose 2B or 4B, a precipitation, with concanavalin A and a recovery in cryoprecipitate. The increase of VIIIR:AG was mostly brought about by the augmentation of relatively slowly migrating portion as demonstrated by crossed immunoelectrophoresis (CIE). Apparently there was no remarkable abnormality in the factor VIII after DDAVP administration, and the elevated factor VIII could function efficiently in vivo. The findings in cryoprecipitation showed that DDAVP could be applied to blood donation for the preparation of cryoprecipitate.