Visualization Principles in Thin-Layer Immunoassays (TIA) on Plastic Surfaces

Abstract

Macromolecules may adsorb firmly as a monolayer to plastic surfaces and still retain their property to react specifically with antibodies. In the present communication four different principles for visualization of such antigen-antibody interactions on plastic surfaces are described. In addition, a diffusion-in-gel method for quantitation of antibodies is presented. The described methods offer simple, sensitive and accurate means of assaying antigen-antibody reactions.