Carbodiimide‐induced protein—RNA crosslinking in mammalian ribosomal subunits
- 8 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 148 (2), 247-250
- https://doi.org/10.1016/0014-5793(82)80817-x
Abstract
RNA—protein interactions in the 60 S subunits of rat liver ribosomes were studied using 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC) under conditions which neither changed the sedimentation coefficient of subunits nor the intactness of their rRNA. EDC induced RNA—protein and protein—protein crosslinkings. Proteins crosslinked to 28 S RNA were identified by two-dimensional gel electrophoreses as L17, L19, L23a and L37a, shown to react with 28 S RNA when using a low dose of UV radiation. Attempts have also been made to use EDC for the studies of RNA—protein interactions in 40 S ribosomal subunitsKeywords
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