Interaction of DNA with DNA‐Binding Proteins
Open Access
- 1 December 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 71 (2), 443-449
- https://doi.org/10.1111/j.1432-1033.1976.tb11132.x
Abstract
DNA-binding protein HD with a monomer MW of 9000 was isolated from E. coli cells. The protein occurs as a tetramer under native conditions and binds to single and double-stranded DNA and to RNA. DNA complexed with protein HD is a poor template for DNA synthesis by E. coli polymerase I, II or III holoenzyme, Exonuclease III is hindered in degrading HD-protein-covered double-stranded DNA, but exonuclease I can digest complexed single-stranded DNA. Transcription is slightly stimulated in the presence of protein HD.This publication has 24 references indexed in Scilit:
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