Expression and Solubilization of a Recombinant Human Neurokinin-1 Receptor in Insect Cells
- 1 January 1994
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 14 (1), 63-73
- https://doi.org/10.3109/10799899409066997
Abstract
The human neurokinin-1 receptor has been expressed in insect cells using a recombinant baculovirus. The expression level is about 10 times higher than that obtained in mammalian cells. The recombinant receptor was solubilized with CHAPS, and a PEG precipitation procedure was shown to be effective in regaining high affinity substance P binding. This system should allow large scale purification of the human neurokinin-1 receptor.Keywords
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