New protein polymorphisms in cattle

Abstract

Preparation techniques are outlined for blood samples from cattle, which permit rapid separation of several constant fractions within a large number of animals. Electrophoretical examination of the blood fractions, the methods for which are described, rendered possible the phenotyping of 5 new polymorphic protein systems. Furthermore the electrophoresis of erythrocyte carboanhydrase was tested. In about 550 cattle, including familial material of the breed 'Deutsche Schwarzbunte', the observed phenotypes, segregation data and allele frequencies of 7 polymorphisms, are described. We were able to find new polymorphisms of serum posttransferrins (Ptf), leucocytic proteins 1 and 2 (Leu1 and Leu2), and erythrocytic proteins 1 and 2 (Ery1, and Ery2). Moreover, data of carbonic anhydrase isoenzymes stained with protein dyes, as well as identified as esterases, are given.