Role of calcium in the programmed death of rat prostatic glandular cells
- 1 January 1990
- journal article
- research article
- Published by Wiley in The Prostate
- Vol. 17 (3), 175-187
- https://doi.org/10.1002/pros.2990170302
Abstract
By using a newly developed and validated rat ventral prostatic organ culture system in which prostatic glandular cells can be induced to undergo programmed cell death, the role of an elevation in the intracellular free Ca2+ concentration in this death process was studied. By using this organ culture system, ventral prostatic glandular epithelial cells can be maintained in culture for a period of more than 14 days with a low daily rate of cell death (i.e., ± 5% die per day) if androgen is included in the media. In contrast, if androgen is not included in the media, the daily rate of prostatic glandular cell death increases approximately 3-fold (i.e., ± 15% die per day). With this organ culture system it has been demonstrated that the daily rate of programmed death of the glandular epithelial cells can be shifted from 5% to 15% of the cells dying per day when testosterone and 10 μM of the Ca2+ ionophore A23187 are both present in the media. Thus, when the intracellular free Ca2+ is elevated within prostatic cells by means of ionophore treatment, the daily rate of glandular cell death in the presence of testosterone is identical to that induced when testosterone is not present in the media. If the organ cultures are maintained in media lacking testosterone but containing 10 μM of the Ca+2 channel blocker nifedipine to inhibit elevations in the intracellular free Ca2+ derived from the extracellular pools, the rise in the daily rate of cell death from 5 to 15% of the cells dying per day induced by androgen ablation can be inhibited by ± 70%. These results suggest that an increase within prostatic glandular cells in their intracellular free Ca+2 derived from extracellular Ca2+ pools is a critical early event involved in triggering the subsequent process of programmed cell death (i.e., specifically DNA fragmentation) in these cells following androgen ablation.Keywords
This publication has 30 references indexed in Scilit:
- Calcium channel antagonists delay regression of androgen‐dependent tissues and suppress gene activity associated with cell deathThe Prostate, 1988
- Activation of a Ca2+−Mg2+‐dependent endonuclease as an early event in castration‐induced prostatic cell deathThe Prostate, 1988
- Characterization and cloning of androgen-repressed mRNAs from rat ventral prostateBiochemical and Biophysical Research Communications, 1987
- Maintenance of adult rat ventral prostate in organ cultureThe Anatomical Record, 1987
- Biological significance of measurable androgen levels in the rat ventral prostate following castrationThe Prostate, 1987
- Quantal relationship between prostatic dihydrotestosterone and prostatic cell content: Critical threshold conceptThe Prostate, 1987
- Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesisThe Journal of Pathology, 1984
- A morphometric analysis of rat ventral prostate in organ cultureThe Anatomical Record, 1983
- Acid Ribonuclease in Rat Prostate During Castration-Induced Involution1Biology of Reproduction, 1980
- Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activationNature, 1980