pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (8), 2721-2728
- https://doi.org/10.1093/nar/5.8.2721
Abstract
I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented. These fragments can serve as accurate DNA size markers from small pieces up to the 4362 base pair length of pBR322. Inserts cloned in this vector may be characterized easily using this data.Keywords
This publication has 8 references indexed in Scilit:
- The cleavage site of the restriction endonuclease Ava IINucleic Acids Research, 1978
- A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilumNucleic Acids Research, 1978
- Detection of 5-methylcytosine in DNA sequencesNucleic Acids Research, 1978
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12Mutation Research, 1975
- Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNAProceedings of the National Academy of Sciences, 1974
- Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresisBiochemistry, 1973