Electrical Activity and Histological Change in the Degenerating Olfactory Epithelium

Abstract

Electrical activity and histological changes were studied in the degenerating olfactory epithelium of the bullfrog after the olfactory nerve had been sectioned. After nerve section, the electrical responses to odors disappeared in the olfactory epithelium in 8 days in the summer, in 11 days in the early autumn, and in 16 days in the early winter. In the degenerating olfactory epithelium a striking decrease in the number of olfactory cells was found, but not of supporting cells. The ratio of the number of olfactory cells to that of supporting cells was found to decrease from 5 or 6 to below 2 after the nerve section. At a ratio below 2, the electrical responses to odor disappeared. The histological changes in the bullfrog are compared with those in the mouse and rabbit. The localization of the olfactory pigment and the electrical activity of the supporting cell are discussed. It was concluded that all three types of responses to odors originate from the activity of the olfactory cell.