Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE
Open Access
- 27 February 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 244 (2), 487-493
- https://doi.org/10.1016/0014-5793(89)80590-3
Abstract
The purpose of this work was to analyze cDNA encoding human monocyte chemoattractant protein‐1 (MCP‐1), previously isolated from glioma cell line culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell line U‐105MG yielded a clone that coded for the entire MCP‐1. Nucleotide sequence analysis and comparison with the amino acid sequence of purified MCP‐1 showed that the cDNA clone comprises a 53‐nucleotide 5′‐non‐coding region, an open reading frame coding for a 99‐residue protein of which the last 76 residues correspond exactly to pure MCP‐1, and a 389‐nucleotide 3′‐untranslated region. The hydrophobicity of the first 23 residues is typical of a signal peptide. Southern blot analysis of human and animal genomic DNA showed that there is a single MCP‐1 gene, which is conserved in several primates. MCP‐1 mRNA was induced in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL‐1, but not by IL‐2, TNF, or IFN‐γ. Among proteins with similar sequences, the coding regions of MCP‐1 and mouse JE show 68% identity. This suggests that MCP‐1 is the human homologue of the mouse competence gene JE.Keywords
This publication has 26 references indexed in Scilit:
- Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties.The Journal of Experimental Medicine, 1988
- Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties.The Journal of Experimental Medicine, 1988
- TGF-β inhibition of endothelial cell proliferation: Alteration of EGF binding and EGF-induced growth-regulatory (competence) gene expressionCell, 1987
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Patterns of Amino Acids near Signal‐Sequence Cleavage SitesEuropean Journal of Biochemistry, 1983
- Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mersJournal of the American Chemical Society, 1983
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976