Electrophoretic karyotype and gene assignment to resolved chromosomes of Trichoderma spp.
- 1 February 1993
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 7 (4), 515-521
- https://doi.org/10.1111/j.1365-2958.1993.tb01142.x
Abstract
A molecular karyotype for three different Trichoderma species (T. harzianum, T. viride, and T. reesei) was determined by using two different systems: countour-clamped electric-field and rotating-electrode electrophoresis. Six chromosomal DNA bands were observed in T. harzianum and T. reesei and five in T. viride. The sizes of these molecules were estimated by their mobility relative to the Schizosaccharomyces pombe chromosomes and ranged between 2.2 and 7.4 megabase pairs (mbp). The estimated genome sizes range from 31 to 39 mbp. A number of genes were located in the different chromosomes by means of Southern analysis. The implications of these findings are discussed.Keywords
This publication has 14 references indexed in Scilit:
- Electrophoretic karyotyping of wild-type and mutant Trichoderma longibrachiatum (reesei) strainsCurrent Genetics, 1992
- Molecular characterization and regulation of the phosphoglycerate kinase gene from Trichoderma virideMolecular Microbiology, 1992
- The use of DNA-fingerprint analysis in the classification of some species of the Trichoderma aggregateCurrent Genetics, 1992
- Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformationCurrent Genetics, 1991
- Molecular Karyotype Analysis of FungiPublished by Elsevier ,1991
- Transformation of Trichoderma viride using the Neurospora crassa pyr4 gene and its use in the expression of a Taka-amylase A gene from Aspergillus oryzaeCurrent Genetics, 1990
- Electrophoretic karyotype of Aspergillus nidulans.Proceedings of the National Academy of Sciences, 1989
- A computer program for choosing optimal oligonudeotides for filter hybridization, sequencing andin vitroamplification of DNANucleic Acids Research, 1989
- Characterization of highly repetitive sequences ofArabidopsis thalianaNucleic Acids Research, 1988
- Identification of ten additional nucleotides in the primary structure of yeast 18S rRNAGene, 1986