Serum Anti-nuclear Factor: Laboratory Studies

Abstract
Anti-nuclear factor (ANF) has been found mainly in the 7S gamma globulin fraction of serum, but in some sera in the macroglobulin also. It is usually removed by absorption with nucleoprotein. Histochemical studies suggest that the reaction between ANF and nuclei requires the presence of both desoxyribonucleic acid (DNA) and histone, probably in combination. Immunization of guinea pigs with nucleoprotein failed to produce a specific immunological response.