Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system
- 13 February 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 167 (1), 165-169
- https://doi.org/10.1016/0014-5793(84)80854-6
Abstract
Twelve bacterial clones containing complementary sequences to yeast tRNAArg 3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT‐3 was mapped, and the presence of a tRNAArg 3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg 3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.Keywords
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