Phorbol ester replacement of helper cell and interleukin 2 requirements in gamma interferon production
- 1 June 1982
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 36 (3), 911-914
- https://doi.org/10.1128/iai.36.3.911-914.1982
Abstract
Immune interferon (IFN-gamma) production by mitogen-stimulated C57BL/6 mouse spleen cells required both Lyt 1+,2- and Lyt 1-,2+ lymphocytes. The Lyt 1-,2+ cells were the IFN-gamma producers, whereas the Lyt 1+,2- cells were the helpers. Interleukin 2 mediated the Lyt 1+,2- cell help. Phorbol myristic acetate (10 ng/ml) completely replaced Lyt 1+,2- helper cell or interleukin 2 requirements in IFN-gamma production. The phorbol ester exerted its helper effects by direct interaction with IFN-gamma-producing Lyt 1-,2+ cells and not through stimulation of production of interleukin 2 by Lyt 1+, 2- cells. Suppressor cell effects in IFN-gamma production were also completely abrogated by phorbol myristic acetate. The data suggest a mechanism for phorbol myristic acetate regulation of IFN-gamma production at the cellular level.This publication has 13 references indexed in Scilit:
- Cellular Regulation of Gamma Interferon Production: Lyt Phenotype of the Suppressor CellInfection and Immunity, 1982
- Biologic properties of chromatographically separated murine thymoma-derived Interleukin 2 and colony-stimulating factor.The Journal of Immunology, 1981
- Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2.The Journal of Immunology, 1981
- Partial purification and characterization of human gamma (immune) interferon.Proceedings of the National Academy of Sciences, 1981
- Cellular regulation of immune interferon productionAntiviral Research, 1981
- Phorbol myristic acetate enhances the production of Interleukin 2Cellular Immunology, 1981
- Thymoma production of T cell growth factor (Interleukin 2).The Journal of Immunology, 1980
- Classification of interferons with antibody to immune interferonCellular Immunology, 1980
- Immune interferon induction by T-cell mitogens involves different T-cell subpopulationsCellular Immunology, 1979
- Large-Scale Production and Partial Purification of Mouse Immune InterferonInfection and Immunity, 1979