Histochemical Demonstration of Acid Phosphatases With Naphthol AS-Phosphates
- 1 September 1958
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 21 (3), 523-538
- https://doi.org/10.1093/jnci/21.3.523
Abstract
A simple, reliable, and convenient azo-dye method for the demonstration of acid phosphatase and phosphamidase in paraffin-embedded tissues is described. The best results are obtained with frozen-dried tissues. Phosphates of naphthols AS-BI, AS-BS, AS-LC, and AS-TR are useful substrates (text-fig. 1, II). The stability and potential high chromogenicity of these substrates suggest their use in quantitative determinations. The present study indicates that acid phosphatase is not as sensitive an enzyme as previously assumed, and that with reasonable care it can be preserved even in mounted sections over a long period.Keywords
This publication has 11 references indexed in Scilit:
- INHIBITION OF PROSTATIC ACID PHOSPHATASE BY α-HYDROXYCARBOXYLIC ACIDSJournal of Biological Chemistry, 1957
- The Cytochemical Localization of EsteraseJNCI Journal of the National Cancer Institute, 1957
- THE VALUE OF FUNDAMENTAL STUDIES OF STAINING REACTIONS IN ENZYME HISTOCHEMISTRY, WITH REFERENCE TO INDOXYL METHODS FOR ESTERASESJournal of Histochemistry & Cytochemistry, 1956
- HISTOCHEMICAL METHODS FOR ACID PHOSPHATASEJournal of Histochemistry & Cytochemistry, 1956
- THE HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE BY A POST-INCUBATION COUPLING TECHNIQUEJournal of Histochemistry & Cytochemistry, 1955
- Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liverBiochemical Journal, 1955
- Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particlesBiochemical Journal, 1955
- Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, β-glucuronidase and cathepsin by rat-liver particlesBiochemical Journal, 1955
- Tissue fractionation studies. 2. The nature of the linkage between acid phosphatase and mitochondria in rat-liver tissueBiochemical Journal, 1951
- INTRACELLULAR LOCALIZATION OF ACID PHOSPHATASEThe Journal of Experimental Medicine, 1951