Assay for Antibody Mediated Cytotoxicity of Mouse Spermatozoa by 86Rubidium Release

Abstract
The analysis of the antigenic structure of murine spermatozoa has been hampered by the lack of a convenient objective serological assay. Spermatozoa, in common with other cells, are capable of concentrating 86Rb. Antibody mediated complement dependent cytotoxicity of murine spermatozoa was quantitated by measuring release of 86Rb from pre-labeled spermatozoa. This assay was used to study the murine T/t locus.