Cell-free translation reconstituted with purified components
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- 1 August 2001
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 19 (8), 751-755
- https://doi.org/10.1038/90802
Abstract
We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).Keywords
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