Regulation of DNA supercoiling in Escherichia coli : Genetic basis of a compensatory mutation in DNA gyrase
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2), 67-70
- https://doi.org/10.1016/0014-5793(89)80931-7
Abstract
Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a topA deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyraseKeywords
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