GABAA and GABAB sites in bovine adrenal medulla membranes
- 31 May 1988
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 20 (2), 241-245
- https://doi.org/10.1002/jnr.490200213
Abstract
The effect of several ligands and Ca2+ ions on [3H]GABA binding to bovine adrenal medulla membranes was investigated. Without any blockade, the [3H]GABA binding showed two components, one of low affinity (Kd = 139 ± 22 nM and Bmax = 3.2 ± 0.4 pmol/mg protein) and the other of high affinity (Kd = 41 ± 6 nM and Bmax = 0.35 ± 0.26 pmol/mg protein). Muscimol specifically blocked low-affinity sites, and ( – )baclofen blocked high-affinity components. Ca2+ ions were strictly necessary for maximum binding to high-affinity sites, whereas they did not significantly affect sites of the lower affinity. These results show that the bovine adrenal medulla has a GABAA receptor population of low affinity together with a GABAB receptor of high affinity.Keywords
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