Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis UW-31 with HeLa cells
- 1 June 1983
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 40 (3), 1060-1067
- https://doi.org/10.1128/iai.40.3.1060-1067.1983
Abstract
Using highly purified elementary bodies of C. trachomatis UW-31 (serotype K), HeLa 229 [human cervical carcinoma] monolayer cultures were found to bind more 32P-labeled chlamydiae after pretreatment with the lectin wheat germ agglutinin. The lectin inhibited competitively when chlamydial association was assayed in the presence of polycations. The 2 effects of wheat germ agglutinin were abolished when N-acetylneuraminic acid (NeuNAc)- or N-acetylglucosamine (GlcNAc)-preincubated wheat germ agglutinin was used. Brief exposure of HeLa cells to neuraminidase abolished the ability to bind the elementary bodies, whether or not polycations were present. At 5.degree. C, but not at 37.degree. C, NeuNAc, GlcNAc and N-acetylgalactosoamine inhibited chlamydial association only in the absence of the polycation DEAE-dextran. Apparently, NeuNAc residues on the plasma membrane are the principal, but not the only, receptors for this strain of C. trachomatis.This publication has 26 references indexed in Scilit:
- Purification of Chlamydia trachomatis Lymphogranuloma Venereum Elementary Bodies and their Interaction with HeLa CellsMicrobiology, 1982
- Attachment of Chlamydia psittaci to Formaldehyde-fixed and Unfixed L CellsMicrobiology, 1981
- Polypeptide Composition of Chlamydia trachomatisMicrobiology, 1981
- Bacterial Adherence: Adhesin-Receptor Interactions Mediating the Attachment of Bacteria to Mucosal SurfacesThe Journal of Infectious Diseases, 1981
- Crystallographic elucidation of the saccharide binding mode in wheat germ agglutinin and its biological significanceJournal of Molecular Biology, 1980
- Sugar‐Lectin Interactions: How Does Wheat‐Germ Agglutinin Bind Sialoglycoconjugates?European Journal of Biochemistry, 1980
- Interaction of wheat germ agglutinin with sialic acidBiochemistry, 1979
- In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stainExperimental Cell Research, 1977
- Lectins: Cell-Agglutinating and Sugar-Specific ProteinsScience, 1972
- Infection of Cell Cultures by Trachoma Agent: Enhancement by DEAE-DextranThe Journal of Infectious Diseases, 1971