Amino Acids in Soil. III. Acids in Hydrolyzates of Water-extracted Soil and Their Distribution in a Pine Forest Soil Profile.

Abstract

Amino acids in hydrolyzates of water-extracted soil were studied by partition chromatography. The soil samples, taken from the F-, H-, and A-layers in a pine forest soil profile from Western Norway, were previously extracted with ether and cold water. The hydrolysis was carried out with 6 N hydrochloric acid at 120[degree]C. The hydrolyzates were evaporated to dryness in vacuo, dissolved in water and desalted on an ionexchange column. 21 ninhydrin-positive spots were observed and of these 16 were quantitatively determined. Inorganic cations in the hydrolyzates have been identified.