Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography
- 1 August 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 16 (2), 355-360
- https://doi.org/10.1128/jcm.16.2.355-360.1982
Abstract
A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.This publication has 10 references indexed in Scilit:
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