Host Cell Factor and an Uncharacterized SANT Domain Protein Are Stable Components of ATAC, a Novel dAda2A/dGcn5-Containing Histone Acetyltransferase Complex in Drosophila
Open Access
- 1 February 2006
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 26 (3), 871-882
- https://doi.org/10.1128/mcb.26.3.871-882.2006
Abstract
Gcn5 is a conserved histone acetyltransferase (HAT) found in a number of multisubunit complexes from Saccharomyces cerevisiae, mammals, and flies. We previously identified Drosophila melanogaster homologues of the yeast proteins Ada2, Ada3, Spt3, and Tra1 and showed that they associate with dGcn5 to form at least two distinct HAT complexes. There are two different Ada2 homologues in Drosophila named dAda2A and dAda2B. dAda2B functions within the Drosophila version of the SAGA complex (dSAGA). To gain insight into dAda2A function, we sought to identify novel components of the complex containing this protein, ATAC (Ada two A containing) complex. Affinity purification and mass spectrometry revealed that, in addition to dAda3 and dGcn5, host cell factor (dHCF) and a novel SANT domain protein, named Atac1 (ATAC component 1), copurify with this complex. Coimmunoprecipitation experiments confirmed that these proteins associate with dGcn5 and dAda2A, but not with dSAGA-specific components such as dAda2B and dSpt3. Biochemical fractionation revealed that ATAC has an apparent molecular mass of 700 kDa and contains dAda2A, dGcn5, dAda3, dHCF, and Atac1 as stable subunits. Thus, ATAC represents a novel histone acetyltransferase complex that is distinct from previously purified Gcn5/Pcaf-containing complexes from yeast and mammalian cells.Keywords
This publication has 50 references indexed in Scilit:
- GCN5: a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genesGene, 2005
- Chromatin remodeling by ATP‐dependent molecular machinesBioEssays, 2003
- Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In VivoMolecular and Cellular Biology, 2001
- The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex PurificationMethods, 2001
- Recruitment of HAT Complexes by Direct Activator Interactions with the ATM-Related Tra1 SubunitScience, 2001
- Mammalian histone acetyltransferases and their complexesCellular and Molecular Life Sciences, 2001
- Structure and function of histone acetyltransferasesCellular and Molecular Life Sciences, 2001
- Large-scale analysis of the yeast proteome by multidimensional protein identification technologyNature Biotechnology, 2001
- Distinct but overlapping roles of histone acetylase PCAF and of the closely related PCAF-B/GCN5 in mouse embryogenesisProceedings of the National Academy of Sciences, 2000
- Loss of Gcn5l2 leads to increased apoptosis and mesodermal defects during mouse developmentNature Genetics, 2000