High-density mapping of single-molecule trajectories with photoactivated localization microscopy
Top Cited Papers
- 13 January 2008
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 5 (2), 155-157
- https://doi.org/10.1038/nmeth.1176
Abstract
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.Keywords
This publication has 14 references indexed in Scilit:
- Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theoriesProceedings of the National Academy of Sciences, 2007
- (Un)Confined Diffusion of CD59 in the Plasma Membrane Determined by High-Resolution Single Molecule MicroscopyBiophysical Journal, 2007
- Plasma Membrane Is the Site of Productive HIV-1 Particle AssemblyPLoS Biology, 2006
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Single-molecule analysis of epidermal growth factor binding on the surface of living cellsThe EMBO Journal, 2006
- Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1The Journal of cell biology, 2006
- Single-Molecule Microscopy Reveals Plasma Membrane Microdomains Created by Protein-Protein Networks that Exclude or Trap Signaling Molecules in T CellsCell, 2005
- Dynamics of putative raft-associated proteins at the cell surfaceThe Journal of cell biology, 2004
- Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm LocalizationScience, 2003
- Constrained diffusion or immobile fraction on cell surfaces: a new interpretationBiophysical Journal, 1996