Coupling Electrophoresis with Ultrafiltration for Improved Processing of Plasma Proteins

Abstract

The feasibility of coupling electrophoresis with ultrafiltration (electroultrafiltration) in order to increase the flux and improve the selectivity of the ultrafiltration process was demonstrated experimentally for protein solutions [bovine serum albumin; .gamma.-globulin]. An electric field controlled the build-up of retained proteins at the membrane surface. Electroultrafiltration fluxes were 3-5 times greater than normal ultrafiltration fluxes. Retention and separation factors were also higher. The effects of important process variables were studied.