Energy expenditure for cyclic retention of NH3/NH4+ during N2 fixation by Klebsiella pneumoniae
- 5 August 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 187 (2), 237-239
- https://doi.org/10.1016/0014-5793(85)81249-7
Abstract
The permeability coefficeint for NH3 through bacterial membranes was determined to be around 2 × 10−3 cm·s−1. This value was used to calculate the outward diffusion of NH3 from intracellular pools and the energy costs for reabsorption as NH4 +. For an N2-fixing continuous culture of Klebsiella pneumoniae an energy expenditure of around 4 ATP per NH3 produced was calculated, thus increasing significantly the energy requirement for N2 fixation in vivo.Keywords
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