Cotranslational heme binding to nascent globin chains
- 1 July 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 326 (1-3), 261-263
- https://doi.org/10.1016/0014-5793(93)81803-8
Abstract
Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain.Keywords
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