The repeating nucleotide sequence in the repetitive mitochondrial DNA from a “low-density” petite mutant of yeast

Abstract

The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethjdjum-jnduced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were used. The sequence consists of 66 consecutive AT base pairs flanked by two GC pairs and comprises nearly all of the mutant mitochondrial genome. The sequence, more over, also represents the first part of wild-type mtDNA sequenced so far.