Photoaffinity Labeling of Chromatin
- 1 February 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 122 (2), 283-289
- https://doi.org/10.1111/j.1432-1033.1982.tb05878.x
Abstract
The syntheses and properties of potential photoaffinity labels for the proteins in chromatin studies are described. One compound, N,N''-bis-(9-acridinyl)-4-aza-4-(4-azidobenzoyl)-1,7-diaminoheptane dihydrochloride, was studied more closely. This photolabel shows high affinity towards [calf thymus] DNA (Ka .apprxeq. 3 .times. 105 M-1) and photoreacts with histones at wavelengths in the range 260-450 nm. The photoreaction was monitored fluorimetrically, and labeling of histones H1, H2A/H2B and H3 was observed. When chromatin was photolabeled, labeling of H1 was exclusively quenched by NaCl at concentrations which are known to cause dissociation of this histone from the DNA. Evidently, only DNA-associated proteins are photolabeled by the reagent.This publication has 26 references indexed in Scilit:
- Entwicklungen in der Arylnitren‐Chemie: Synthesen und MechanismenAngewandte Chemie, 1979
- The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activityCell, 1979
- INTERACTION OF 9‐AMINOACRIDINE WITH 7‐METHYLGUANOSINE AND 1,N6‐ETHENOADENOSINE MONOPHOSPHATEPhotochemistry and Photobiology, 1978
- ChromatinNature, 1978
- Structure of ChromatinAnnual Review of Biochemistry, 1977
- Relaxation of chromatin structure by ethidium bromide binding: determined by viscometry and histone dissociation studiesBiochemistry, 1976
- Chromosomal Proteins and Chromatin StructureAnnual Review of Biochemistry, 1975
- Histone-histone associations within chromatin. Crosslinking studies using tetranitromethaneBiochemistry, 1975
- Photosensitive azide polymersJournal of Applied Polymer Science, 1963
- Structural considerations in the interaction of DNA and acridinesJournal of Molecular Biology, 1961